Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA
Possible
RMgm-4846 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-4846 Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-4846
Normal gametocyte production. Only a few microgametocytes formed active exflagellation centres after activation. Severe reduction in exflagellation. The defect was already apparent at 1 min pa, with a >3 fold reduction of cells with more than 2N DNA content and a 3-fold reduction in mitotic spindles.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

PfHsp110c prevents the aggregation of Asn-rich protein during febrile episodes. Live fluorescence images of PfHsp110c–RFA parasite lines expressing (a,b) the Asn repeat-rich parasite protein, PFI1155w. incubated at 400C for 6 h with (a) or without (b) 10mM TMP. The arrowheads indicate fluorescent foci of PFI1155w–tagRFP-T (b). Scale bar represents 2 mm. Images from left to right are phase, DAPI, GFP (MAL13P1.540), RFP (PFI1155w) and GFP–RFP merge. Parasites that were subjected to heat shock without TMP showed foci of PFI1155w–tagRFP-T (b) and indicative of protein. The distribution of PfHsp110c–RFA also was more focal upon heat shock in the absence of TMP (b) in the absence of TMP is specifically owing to the loss of PfHsp110c function.Muralidharan V, Oksman A, Pal P, Lindquist S, Goldberg DE. Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers. Nat Commun. 2012 Dec 18;3:1310.

See original on MMP

PfHsp110c prevents the aggregation of Asn-rich protein during febrile episodes. Live fluorescence images of PfHsp110c–RFA parasite lines expressing (a,b) the Asn repeat-rich parasite protein, PFI1155w. incubated at 400C for 6 h with (a) or without (b) 10mM TMP. The arrowheads indicate fluorescent foci of PFI1155w–tagRFP-T (b). Scale bar represents 2 mm. Images from left to right are phase, DAPI, GFP (MAL13P1.540), RFP (PFI1155w) and GFP–RFP merge. Parasites that were subjected to heat shock without TMP showed foci of PFI1155w–tagRFP-T (b) and indicative of protein. The distribution of PfHsp110c–RFA also was more focal upon heat shock in the absence of TMP (b) in the absence of TMP is specifically owing to the loss of PfHsp110c function.Muralidharan V, Oksman A, Pal P, Lindquist S, Goldberg DE. Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers. Nat Commun. 2012 Dec 18;3:1310.

See original on MMP

More information