Last updated 5 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
30728298
(Crispr-assisted). Var genes silenced, alongside other transcriptomic changes. 'deletion of PfRecQ1 increased the heterochromatin mark trimethylated (H3K9me3) at the transcription start site (TSS) of the var gene upsC1, that deletion had no effect on the global distribution of H3K9me3 over gene bodies, including those for the var genes'. Rescued by complementation.
Theo Sanderson, Google AI
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
26917473 (Knock down) Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Asexual
Transcriptional effect
30728298
(Crispr-assisted). Var genes silenced, alongside other transcriptomic changes. 'deletion of PfRecQ1 increased the heterochromatin mark trimethylated (H3K9me3) at the transcription start site (TSS) of the var gene upsC1, that deletion had no effect on the global distribution of H3K9me3 over gene bodies, including those for the var genes'. Rescued by complementation.
Theo Sanderson, Google AI

Imaging data (from Malaria Metabolic Pathways)

Localization of PfBlm in different intra-erythrocytic stages of P. falciparum by immunofluorescence staining and confocal microscopy. The cells were fixed and immunostained with purified primary antibodies against PfBlm followed by fluoro-isothiocyanate-labelled secondary antibody and then counterstained with DAPI. In each panel, single confocal image of each stage is shown. (a) Ring stage, (b) Trophozoite stage, (c) Early schizont stage, (d) Schizont stage, (e) Double infection in schizont stage, (f) Bursting schizont stage. In each panel (i) phase contrast image; (ii) image of cell stained with DAPI (blue); (iii) immunofluorescently stained cell (green); (iv) super-imposed image; (v) merged.Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom omologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 Feb 23. [Epub ahead of print]

See original on MMP

Co-localization of PfBlm and PfWrn by immunofluorescence dual colour staining and confocal microscopy. Panels a, b, c, d and e show the images of merozoite attached to RBC, trophozoite, early schizont, mature schizont and late schizont respectively. (i) Immunofluorescently stained cell (green, P. falciparum Wrn protein), (ii) immunofluorescently stained cell (red, PfBlm), (iii) super-imposed images of PfBlm and PfWrn, (iv) super-imposed images. The results reveal that PfWrn was found in nucleus as reported and PfBlm is expressed in nucleus as well as in cytoplasm. The co-localization of PfWrn and PfBlm was observed to some extent in invading merozoite (a) and trophozoite stages.Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom homologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 May;1864(5):594-608

See original on MMP

Localization of PfBlmin different intra-erythrocytic stages of P. falciparum by immunofluorescence staining and confocalmicroscopy. The cells were fixed and immunostained with purified primary antibodies against PfBlm followed by fluoroisothiocyanate-labelled secondary antibody and then counterstained with DAPI. In each panel, single confocal image of each stage is shown. (a) Ring stage, (b) trophozoite stage, (c) early schizont stage, (d) schizont stage, (e) double infection in schizont stage, (f) bursting schizont stage. In each panel (i) phase contrast image; (ii) image of cell stained with DAPI (blue); (iii) immunofluorescently stained cell (green); (iv) super-imposed image; (v) merged. These data are representative of threeseparate experiments. The results showthat Blm protein expresses and localizesmainly in the nuclear region during the ring and trophozoite stages (a–b) but it is also detectable in cytoplasm during the schizont stages of intraerythrocytic development of the parasite.Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom homologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 1864(5):594-608.

See original on MMP

More information

PlasmoDB PF3D7_0918600
GeneDB PF3D7_0918600
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PFI0910w
Orthologs PBANKA_0819500 , PCHAS_0819800 , PKNH_0716600 , PY17X_0822800
Google Scholar Search for all mentions of this gene