Last updated 3 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
18069893 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
33713652
Our results showed a change in expression of redox regulators in the apicoplast and the cytosol. We further detected a change in parasite central carbon metabolism, with lipB deletion resulting in changes to glycolysis and tricarboxylic acid cycle activity. Further, in another Plasmodium cell line (NF54), deletion of lipB impacted development in the mosquito, preventing the detection of infectious sporozoite stages
Theo Sanderson, Francis Crick Institute
P. berghei ANKA
Possible
RMgm-851 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
18069893 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Asexual
Difference from wild-type
33713652
Our results showed a change in expression of redox regulators in the apicoplast and the cytosol. We further detected a change in parasite central carbon metabolism, with lipB deletion resulting in changes to glycolysis and tricarboxylic acid cycle activity. Further, in another Plasmodium cell line (NF54), deletion of lipB impacted development in the mosquito, preventing the detection of infectious sporozoite stages
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Sporozoite
Attenuated
33713652
Our results showed a change in expression of redox regulators in the apicoplast and the cytosol. We further detected a change in parasite central carbon metabolism, with lipB deletion resulting in changes to glycolysis and tricarboxylic acid cycle activity. Further, in another Plasmodium cell line (NF54), deletion of lipB impacted development in the mosquito, preventing the detection of infectious sporozoite stages
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
No difference
RMgm-851 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Oocyst
No difference
RMgm-851 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-851 Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-851
Reduced infectivity of sporozoites. Mice infected with sporozoites show a 4 day delay in prepatent period. Mutant liver stages have major defects during late liver stage development and do not produce detached cells during in vitro culture.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Localization of LipA–GFP fusion and LplA–GFP fusion constructs overexpressed in P. falciparum. A. The first N-terminal 339 nucleotides of the lipA gene were cloned in frame with green fluorescent protein (GFP). The peptide confers transit of GFP into an organelle distinct from the mitochondrion as shown by co-staining the parasites with MitoTracker CMX Ros. The figure demonstrates the morphological changes of the apicoplast throughout the erythrocytic cycle of the parasites. Phase: phase-contrast image MitoTracker: image. Merge: merge of phase-contrast, GFP and rhodamine channel. B. The first N-terminal 357 nucleotides of the lplA-GFP targets to the mitochondrion. The co-localization of GFP and MitoTracker fluorescence suggests that the LplA N-terminal extension represents mitochondrial targeting sequence. taken of mitochondrion using the rhodamine channel.Wrenger C, Müller S. The human malaria parasite Plasmodium falciparum has distinct organelle-specific lipoylation pathways. Mol Microbiol. 2004 53:103-13.

See original on MMP

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