Last updated 1 year ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
22849771 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA
Possible
RMgm-257 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-60 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-59 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) Imported from PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
22849771 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Gametocyte
No difference
22849771 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
No difference
RMgm-257 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-60 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-59 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) Imported from PlasmoGEM
P. berghei ANKA Gametocyte
No difference
RMgm-60 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-59 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-60
Gamete formation is not affected. Macrogametes become activated but fail to develop into ookinetes.Fertilisation occurs, but development is arrested at 2N zygote stage. The defect is female gamete specific; male gametes are fertile and able to fertilize wild-type female gametes.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-59
Gamete formation is not affected. Fertilisation occurs, but development is arrested at 2N zygote stage. The defect is female gamete specific; male gametes are fertile and able to fertilize wild-type female gametes.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Episomal expression of GFP-tagged Pfnek-4 protein multinucleated-schizont stage from transgenic 3D7 parasites expressing GFP-tagged Pfnek-4 controlled by cognate (Pfnek-4) promoter. Overlay between the green and blue channels, and corresponding DIC images are shown as well. Scale bars, 2.0 μm. While the bulk of examined schizonts did not show any detectable signal, as expected for a protein thought to be gametocyte-specific, fluorescence microscopy of asexual stage cultures consistently identified a small subpopulation of schizonts with dots (single or doublets) of concentrated Pfnek-4-GFP protein associated with nuclei. Fluorescence was never detected in ring and trophozoite stages. Noteworthy, all nuclei within a single schizont appeared to be associated with punctuate Pfnek-4-GFP fluorescence from early developing schizont to multinucleated schizonts (right). Reininger L, Garcia M, Tomlins A, Müller S, Doerig C. The Plasmodium falciparum, Nima-related kinase Pfnek-4: a marker for asexual parasites committed to sexual differentiation. Malar J. 2012 Jul 31;11(1):250.

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Transgenic expression of GFP-tagged Pfnek-4 and Pfnek-2 proteins in P. falciparum parasites. Live images of Hoechst-stained P. falciparum 3D7 transfectants episomally expressing Pfnek-2-GFP (A) or Pfnek-4-GFP (B) proteins under cognate Pfnek-2 and Pfnek-4 promoters, respectively. A. The Pfnek-2-GFP fluorescence (green) is not observed in stage II gametocytes (G-II), but appears to be associated to microtubule structures in more mature stage IV gametocytes (G-IV). B. The Pfnek-4-GFP fluorescence (green) appears as single dots associated with Hoechst-stained nuclear bodies (blue) in a subset of schizonts (S) and stage II gametocytes (G-II). DIC images are shown as well.Doerig C, Reininger L. Nima- and Aurora-related kinases of malaria parasites. Biochim Biophys Acta. 2013 1834(7):1336-45

See original on MMP

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