Last updated 1 month ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
29507322
(With Cas9). "Whereas controls using analogous plasmids targeting other regions generated viable parasites, no parasites harbouring a truncated version of PfGNA1 could be obtained (Fig. 7A). This suggests that the PfGNA1 gene plays an essential role for the survival of the parasite. In a second set of experiments, we were also unable to introduce nonsense mutations in the N-terminus of the GNAT domain conserved region. In contrast, in three independent biological replicates carried out in parallel, we successfully managed to obtain viable parasites harbouring synonymous nucleotide substitutions in the same region of the GNAT conserved domain (Fig. 7C). Therefore, nucleotides in the catalytic GNAT domain of PfGNA1 are genetically modifiable as long as the open reading frame is not altered. These data strongly suggest that an unaltered version of the PfGNA1 protein is required for parasite viability, at least during P. falciparum asexual blood stages."
Theo Sanderson, Google AI
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

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More information

PlasmoDB PF3D7_0629000
GeneDB PF3D7_0629000
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2270.t00178, MAL6P1.138, PFF1405c
Orthologs PBANKA_1127600 , PCHAS_1127100 , PKNH_1120800 , PVP01_1120500 , PVX_114550 , PY17X_1129100
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