Last updated 1 year ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
18346224 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA
Possible
RMgm-621 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-144 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-143 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) Imported from PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
18346224 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
No difference
RMgm-621 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-143 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-144 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-621 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-143 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-144 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-621 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-143 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-144 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-621 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-143 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-144 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-621
Mutant sporozoites show impaired motility and reduced speed. The prepatency period was prolonged by one day when mutant sporozoites were injected by mosquito bite or subcutaneously. No delay in blood stage infection was observed when sporozoites were injected intravenously.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-143
Sporozoites showed normal gliding motility.Sporozoites showed a twofold decrease in infectivity compared with wild type after infection of mice by intravenous injection. The sporozoites showed a 20-fold decrease in infectivity when mice were infected by intradermal inoculation.The level of HepG2 invasion in vitro of mutant sporozoites was similar to wild type sporozoites and invaded parasites developed efficiently into mature schizonts. In vitro migration/cell traversal assays using Hepa 16 cells and mouse dermal fibroblasts (MDFs) showed a twofold reduction in the migratory activity of sporozoites compared with wild type. Treatment with the cysteine protease inhibitor E-64d, which prevents invasion and results in maximally migratory sporozoites, increased the cell traversal activity but not to the level that was observed with wild type sporozoites.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-144
Normal numbers of midgut (oocyst-derived) and salivary gland sporozoites are produced. Sporozoites showed normal gliding motility, albeit at a lower frequency. Sporozoites showed full in vitro and in vivo infectivity in cultured hepatoma cells and when injected intravenously into rats, respectively.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-621
The prepatency period was prolonged by one day when mutant sporozoites were injected by mosquito bite or subcutaneously. No delay in blood stage infection was observed when sporozoites were injected intravenously.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-144
Sporozoites showed full in vitro and in vivo infectivity in cultured hepatoma cells and when injected intravenously into rats, respectively.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-143
Sporozoites showed a twofold decrease in infectivity compared with wild type after infection of mice by intravenous injection. The sporozoites showed a 20-fold decrease in infectivity when mice were infected by intradermal inoculation.The level of HepG2 invasion in vitro of mutant sporozoites was similar to wild type sporozoites and invaded parasites developed efficiently into mature schizonts. In vitro migration/cell traversal assays using Hepa 16 cells and mouse dermal fibroblasts (MDFs) showed a twofold reduction in the migratory activity of sporozoites compared with wild type. Treatment with the cysteine protease inhibitor E-64d, which prevents invasion and results in maximally migratory sporozoites, increased the cell traversal activity but not to the level that was observed with wild type sporozoites.
Imported from RMgmDB

More information

PlasmoDB PF3D7_0616500
GeneDB PF3D7_0616500
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2270.t00053, MAL6P1.261, PFF0800w
Orthologs PBANKA_1116000 , PCHAS_1115600 , PKNH_1133500 , PVP01_1132600 , PVX_113965 , PY17X_1117200
Google Scholar Search for all mentions of this gene