Last updated 1 month ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
22127061 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
36154191
\'In contrast to previous findings, we showed that gene deletion Pfcrk5- parasites grew normally as asexual stages and underwent normal gametocytogenesis to stage V gametocytes. However, Pfcrk5- parasites showed a severe defect in male gametogenesis, which was evident by a significant reduction in the emergence of male gametes (exflagellation). This defect caused a severe reduction of parasite transmission to the mosquito. Genetic crosses performed using sex-specific sterile transgenic parasites revealed that Pfcrk5- parasites suffered a defect in male fertility but female gametes were fertile. Taken together, these results demonstrate that PfCRK5 is a critical sexual stage kinase which regulates male gametogenesis and transmission to the mosquito.\'
Theo Sanderson, Francis Crick Institute
P. berghei ANKA
Refractory
RMgm-556 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-4845 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
36154191
\'In contrast to previous findings, we showed that gene deletion Pfcrk5- parasites grew normally as asexual stages and underwent normal gametocytogenesis to stage V gametocytes. However, Pfcrk5- parasites showed a severe defect in male gametogenesis, which was evident by a significant reduction in the emergence of male gametes (exflagellation). This defect caused a severe reduction of parasite transmission to the mosquito. Genetic crosses performed using sex-specific sterile transgenic parasites revealed that Pfcrk5- parasites suffered a defect in male fertility but female gametes were fertile. Taken together, these results demonstrate that PfCRK5 is a critical sexual stage kinase which regulates male gametogenesis and transmission to the mosquito.\'
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Gametocyte
Attenuated
36154191
\'In contrast to previous findings, we showed that gene deletion Pfcrk5- parasites grew normally as asexual stages and underwent normal gametocytogenesis to stage V gametocytes. However, Pfcrk5- parasites showed a severe defect in male gametogenesis, which was evident by a significant reduction in the emergence of male gametes (exflagellation). This defect caused a severe reduction of parasite transmission to the mosquito. Genetic crosses performed using sex-specific sterile transgenic parasites revealed that Pfcrk5- parasites suffered a defect in male fertility but female gametes were fertile. Taken together, these results demonstrate that PfCRK5 is a critical sexual stage kinase which regulates male gametogenesis and transmission to the mosquito.\'
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
No difference
RMgm-4845 Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-4845
Normal gametocyte production. Only a few microgametocytes formed active exflagellation centres after activation.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-4845
CRK5-KO parasites showed a significant reduction in ookinete conversion compared to the parental control line
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4845
A > 30 fold decrease in the number of CRK5-KO oocysts present oin A. stephensi mosquitoes allowed to feed on infected mice. Oocysts showed aberrant morphology. No formation of viable sporozoites. No blood stage infection developed in mice after infection by bite of infected mosquitoes.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-4845
A > 30 fold decrease in the number of CRK5-KO oocysts present in A. stephensi mosquitoes allowed to feed on infected mice. Oocysts showed aberrant morphology. No formation of viable sporozoites. No blood stage infection developed in mice after infection by bite of infected mosquitoes.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-4845
A > 30 fold decrease in the number of CRK5-KO oocysts present in A. stephensi mosquitoes allowed to feed on infected mice. Oocysts showed aberrant morphology. No formation of viable sporozoites. No blood stage infection developed in mice after infection by bite of infected mosquitoes.
Imported from RMgmDB

More information

PlasmoDB PF3D7_0615500
GeneDB PF3D7_0615500
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2270.t00045, MAL6P1.271, PFF0750w
Orthologs PBANKA_1230200 , PCHAS_1230900 , PKNH_1134500 , PVP01_1133600 , PVX_113910 , PY17X_1233700
Google Scholar Search for all mentions of this gene