Last updated 2 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
20195509 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. yoelii yoelii 17X
Refractory
RMgm-4381 Imported from RMgmDB

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

PfSIP2-N localizes to P. falciparum chromosome end clusters. (A) IFA detects discrete perinuclear PfSIP2-N-HA signals in late trophozoites (LT) and early (ES) and late (LS) schizont stage 3D7/SIP2-N-HA parasites. The expression cassette is schematically depicted on the left. (B) Co-localisation of PfSIP2-N with PfHP1-containing subtelomeric heterochromatin in late trophozoites (LT) and early (ES) and late (LS) schizonts in the double transgenic parasite line 3D7/SIP2-N-HA/HP1-Ty (over-expressing both proteins as epitope-tagged versions simultaneously). Expression cassettes are schematically depicted on the left. Indirect immunofluorescence (IFA) microscopy identified discrete PfSIP2-N-HA foci at the nuclear periphery with increasing numbers of foci in replicating stages (A) consistent with chromosome end clusters. PfHP1 is a heterochromatic marker. Both proteins co-localized at the nuclear periphery (B). Flueck C, Bartfai R, Niederwieser I, Witmer K, Alako BT, Moes S, Bozdech Z, Jenoe P, Stunnenberg HG, Voss TS. A major role for the Plasmodium falciparum ApiAP2 protein PfSIP2 in chromosome end biology. PLoS Pathog. 2010 6:e1000784.

See original on MMP

More information

PlasmoDB PF3D7_0604100
GeneDB PF3D7_0604100
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2270.t00272, MAL6P1.44, PFF0200c
Orthologs PBANKA_0102900 , PCHAS_0103600 , PKNH_1146400 , PVP01_1144800 , PVX_113370 , PY17X_0104500
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