Last updated 1 month ago

Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
23041242 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
32815516 (Conditional)
Conditional system in which mutant was generated with supplementation so that apicoplast is not required. "Upon removal of mevalonate both deletion lines failed to grow "
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
No difference
23041242 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Oocyst
Difference from wild-type
23041242 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Sporozoite
Difference from wild-type
23041242 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Liver
Difference from wild-type
23041242 Theo Sanderson, Wellcome Trust Sanger Institute

Imaging data (from Malaria Metabolic Pathways)

Immunolocalization of PfoTPT to the outside of free, intact apicoplasts. (A) Z-stack of immunofluorescent images (1– 8) showing localization of anti-PfoTPT (red) around apicoplast-targeted GPF (green) in parasites expressing PfACP(leader)-GFP. (B) Electron micrograph of intact apicoplast labeled with anti-PfoTPT antibodies and 20-nm colloidal gold. (C) Tannic acid fixation showing four bounding membranes around isolated apicoplast labeled with anti-PfoTPT antibodies and 10-nm colloidal gold. (Scale bars, 200 nm.)Lowest row: Anti-PfiTPT (red) colocalizes with apicoplast-targeted GPF (green) in parasites expressing PfACP(leader)-GFP.Mullin KA, Lim L, Ralph SA, Spurck TP, Handman E, McFadden GI. Membrane transporters in the relict plastid of malaria parasites. Proc Natl Acad Sci U S A. 2006 103:9572-7. Copyright 2009 National Academy of Sciences, U.S.A.

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Indirect immunofluorescence assay on TPT–GFP parasites: TPT–GFP blood stage parasites were stained with primary rabbit anti-ENR polyclonal antibody and visualized by staining with secondary anti-rabbit IgG antibody coupled with Alexa 594. Parasite nuclei are stained with DAPI. In DIC are shown parasitized RBC. Merge shows overlap of TPT–GFP, rabbit anti-enoyl ACP reductase (ENR), DAPI and DIC. Apicoplast area is zoomed to show the colocalization of TPT–GFP with ENR. TPT, which was correctly localized to apicoplast. Plasmodium enoyl ACP reductase (ENR) is well-known apicoplast resident protein.Banerjee T, Jaijyan DK, Surolia N, Singh AP, Surolia A. Apicoplast triose phosphate transporter (TPT) gene knockout is lethal for Plasmodium. Mol Biochem Parasitol. 2012 186(1):44-50. . Copyright Elsevier.

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More information

PlasmoDB PF3D7_0530200
GeneDB PF3D7_0530200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL5P1.299, PFE1510c
Orthologs PBANKA_1244600 , PCHAS_1245000 , PKNH_1002500 , PVP01_1003700 , PVX_079755 , PY17X_1248100
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