Last updated 3 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
22127061 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
RMgm-294 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-288 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-294 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-288 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-294 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-288 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-294
Male (micro) gametocytes are able to exflagellate as efficiently as wild-type parasites and female (macro)gametocytes emerge normally. Fertilisation occurs, but development is arrested at the 2N zygote stage. The defect is female gamete specific; male gametes are fertile and able to fertilize wild-type female gametes.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-288
Male (micro) gametocytes are able to exflagellate as efficiently as wild-type parasites and female (macro)gametocytes emerge normally. Fertilisation occurs, but development is arrested at the 2N zygote stage. The defect is female gamete specific; male gametes are fertile and able to fertilize wild-type female gametes.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-288
Fertilisation of female gametes occurs, but development is arrested at the 2N zygote stage and no mature ookinetes are formed. No oocyst are formed in Anopheles stephensi mosquitoes.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-294
Fertilisation of female gametes occurs, but development is arrested at the 2N zygote stage and no mature ookinetes are formed. No oocyst are formed in Anopheles stephensi mosquitoes.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Transgenic expression of GFP-tagged Pfnek-4 and Pfnek-2 proteins in P. falciparum parasites. Live images of Hoechst-stained P. falciparum 3D7 transfectants episomally expressing Pfnek-2-GFP (A) or Pfnek-4-GFP (B) proteins under cognate Pfnek-2 and Pfnek-4 promoters, respectively. A. The Pfnek-2-GFP fluorescence (green) is not observed in stage II gametocytes (G-II), but appears to be associated to microtubule structures in more mature stage IV gametocytes (G-IV). B. The Pfnek-4-GFP fluorescence (green) appears as single dots associated with Hoechst-stained nuclear bodies (blue) in a subset of schizonts (S) and stage II gametocytes (G-II). DIC images are shown as well.Doerig C, Reininger L. Nima- and Aurora-related kinases of malaria parasites. Biochim Biophys Acta. 2013 1834(7):1336-45

See original on MMP

More information

PlasmoDB PF3D7_0525900
GeneDB PF3D7_0525900
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL5P1.258, PFE1290w
Orthologs PBANKA_1240700 , PCHAS_1241100 , PKNH_1006900 , PVP01_1007900 , PVX_079950 , PY17X_1243900
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