Last updated 8 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Co-localization of the TMD protein MAL13P1.130 and the alveolin PFE1285w with the disassembling spindle apparatus during schizogony (T1-5). Upper: While the mitotic spindles (T1, anti-tubulin, red) are still present, the cramp-like structure highlighted by the TMD protein MAL13P1.130-GFP is formed (T2). Lower: In contrast, the alveolin-defined structure only emerges after the spindles have retracted forming MTOC bundles (T3). Nuclei stained with DAPI. Scale bars, 2μm.Kono M, Herrmann S, Loughran NB, Cabrera A, Engelberg K, Lehmann C, Sinha D, Prinz B, Ruch U, Heussler V, Spielman T, Parkinson J, Gilberger TW. Evolution and Architecture of the Inner Membrane Complex in Asexual and Sexual Stages of the Malaria Parasite. Mol Biol Evol. 2012 29(9):2113-32.

See original on MMP

Group B IMC associated proteins reveal distinct dynamics. A. The GFP-tagged alveolins PF10_0039, PFE1285w and MAL13P1.228 emerge as large ring-like structures (T1-2) that towards the end of schizogony show the same subpellicular distributions as their TMD counterparts (T3).Kono M, Herrmann S, Loughran NB, Cabrera A, Engelberg K, Lehmann C, Sinha D, Prinz B, Ruch U, Heussler V, Spielman T, Parkinson J, Gilberger TW. Evolution and Architecture of the Inner Membrane Complex in Asexual and Sexual Stages of the Malaria Parasite. Mol Biol Evol. 2012 29(9):2113-32.

See original on MMP

Upper panel: Co-localization of the group A protein PFD1110w (anti-PFD1110w, red) with the group B alveolin PFE1285w (PFE1285w-GFP, green) emphasizes the differential nature of the two structures in the nascent IMC and their spatial relation during the ongoing schizogony (T1-3). Nuclei stained with DAPI. Scale bar 2 μm.Lower panel: A double transgenic parasite line was generated expressing a different combination of proteins, MAL13P1.130-GFP (group A) and PF10_0039-mCherry. The dynamic distribution of these two members confirmed the spatial distinction but intimate proximity of the group A and group B defined IMC sub-compartments during the early phase of IMC biogenesis.Kono M, Herrmann S, Loughran NB, Cabrera A, Engelberg K, Lehmann C, Sinha D, Prinz B, Ruch U, Heussler V, Spielman T, Parkinson J, Gilberger TW. Evolution and Architecture of the Inner Membrane Complex in Asexual and Sexual Stages of the Malaria Parasite. Mol Biol Evol. 2012 29(9):2113-32

See original on MMP

Subcellular distribution of proteins predicted to be involved in invasion. All proteins were localized in schizonts (s) and free merozoites (m) using GFP-fusion proteins and grouped according to their predominant GFP localization: surface (green), inner membrane complex (turquoise),Hu G, Cabrera A, Kono M, Mok S, Chaal BK, Haase S, Engelberg K, Cheemadan S, Spielmann T, Preiser PR, Gilberger TW, Bozdech Z. Transcriptional profiling of growth perturbations of the human malaria parasite Plasmodium falciparum. Nat Biotechnol. 2010 28(1):91-8.

See original on MMP

More information

PlasmoDB PF3D7_0525800
GeneDB PF3D7_0525800
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL5P1.257, PFE1285w
Orthologs PBANKA_1240600 , PCHAS_1241000 , PKNH_1007000 , PVP01_1008000 , PVX_079955 , PY17X_1243800
Google Scholar Search for all mentions of this gene