Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Refractory
RMgm-4584 Imported from RMgmDB

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Spatial distribution of P. falciparum Ap4AH during erythrocytic schizogony. Shown are DAPI staining of nucleus in blue and PfAp4AH stained with Alexa 488 in green. (A) Confocal microscopy-data based spatial distribution of PfAp4AH in infected RBCs. PfAp4AH is non-nuclear in blood stages of the parasite and resides in its cytoplasm. (B) Non-mitochondrial localization with various controls is shown. Upper panel shows pre-immune serum (Pre-Imm Sera) control which does not stain the parasite or RBCs. Middle panel shows competitive binding of anti-PfAp4AH antibody to infected cells, where anti-PfAp4AH antibodies were incubated with recombinant PfAp4AH protein in 1:5 ratio. Pf D-tyrosyl-tRNATyr deacylase (DTD) is a cytoplasmic marker. Lower panel shows non-mitochondrial localization where mtochondria are stained in red. (C) RBC membrane localization of PfAp4AH during trophozoite and schizont stages of parasite. VarC is a marker for infected RBC membrane localization. (D) A field view of anti-PfAp4AH antibody staining of infected RBCs. Significant fraction of cells (~50%) showed membrane localization of PfAp4AH - here cell is marked with white arrow. Uninfected RBCs (without DAPI and PfDTD staining here) are unstained. White scale bar in confocal figures is of 5 μmSharma A, Yogavel M, Sharma A. Structural and functional attributes of malaria parasite diadenosine tetraphosphate hydrolase. Sci Rep. 2016 Feb 1;6:19981.

See original on MMP

More information

PlasmoDB PF3D7_0520600
GeneDB PF3D7_0520600
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL5P1.207, PFE1035c
Orthologs PBANKA_1235300 , PCHAS_1235900 , PKNH_1012300 , PVP01_1013300 , PVX_080215 , PY17X_1238700
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