Last updated 1 month ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
27383769
\'PfPuf1 disruption did not interfere with the asexual erythrocyte cycle of the parasite but resulted in an approximately tenfold decrease of mature gametocytes. In the PfPuf1-disrupted lines, gametocytes appeared normal before stage III but subsequently exhibited a sharp decline in gametocytemia. This was accompanied by a concomitant accumulation of dead and dying late-stage gametocytes, which retained normal gross morphology. In addition, significantly more female gametocytes were lost in the PfPuf1-disrupted lines during development, resulting in a reversed male-to-female sex ratio.\'
Theo Sanderson, Francis Crick Institute
P. berghei ANKA
Possible
RMgm-616 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-514 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-513 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
27383769
\'PfPuf1 disruption did not interfere with the asexual erythrocyte cycle of the parasite but resulted in an approximately tenfold decrease of mature gametocytes. In the PfPuf1-disrupted lines, gametocytes appeared normal before stage III but subsequently exhibited a sharp decline in gametocytemia. This was accompanied by a concomitant accumulation of dead and dying late-stage gametocytes, which retained normal gross morphology. In addition, significantly more female gametocytes were lost in the PfPuf1-disrupted lines during development, resulting in a reversed male-to-female sex ratio.\'
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Gametocyte
Attenuated
27383769
\'PfPuf1 disruption did not interfere with the asexual erythrocyte cycle of the parasite but resulted in an approximately tenfold decrease of mature gametocytes. In the PfPuf1-disrupted lines, gametocytes appeared normal before stage III but subsequently exhibited a sharp decline in gametocytemia. This was accompanied by a concomitant accumulation of dead and dying late-stage gametocytes, which retained normal gross morphology. In addition, significantly more female gametocytes were lost in the PfPuf1-disrupted lines during development, resulting in a reversed male-to-female sex ratio.\'
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
No difference
RMgm-616 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-514 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-513 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-616 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-514 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-513 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-616 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-514
Normal numbers of gametocytes and ookinetes are produced (see also 'Additional remarks phenotype').
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-513
Normal numbers of gametocytes and ookinetes are produced (see also 'Additional remarks phenotype').
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-616
Lower numbers of oocysts and salivary gland sporozoites compared to wild type (although not significant!)
Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-514 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-513 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-616
Lower numbers of oocysts and salivary gland sporozoites compared to wild type (although not significant!). Sporozoites are infective to mice although parasite liver loads after intravenous inoculation of sporozoites are 4x reduced compared to wild type. Sporozoites showed normal invasion of and development in HepG2 cells in vitro.
Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-514 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-513 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-616 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-514 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-513 Imported from RMgmDB

More information

PlasmoDB PF3D7_0518700
GeneDB PF3D7_0518700
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL5P1.187, PFE0935c
Orthologs PBANKA_1233500 , PCHAS_1234100 , PVP01_1015200 , PVX_080310 , PY17X_1236900
Google Scholar Search for all mentions of this gene