Last updated 1 year ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
22151036 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Localization of Plasmodium falciparum c-glutamylcysteine synthetase–green fluorescent protein (PfcGCSGFP) and glutathione synthetase-GFP (PfGS-GFP). P. falciparum GS and gGCS C-terminally tagged with GFP were expressed in P. falciparum D10 erythrocytic stages. Fluorescence was analyzed using an Axioskop-2 mot plus microscope (Zeiss) equipped with a Hamamatsu C4742-95 CCD camera and was shown to be present throughout the cytoplasm of both transfected parasite lines, suggesting that GSH biosynthesis is cytoplasmic. No fluorescence is seen in the digestive vacuoles (DV) of the parasites or the erythrocyte host cell (E). E, erythrocyte; P, parasite; DV, digestive vacuole with hemozoin crystals.Patzewitz EM, Salcedo-Sora JE, Wong EH, Sethia S, Stocks PA, Maughan SC, Murray JA, Krishna S, Bray PG, Ward SA, Müller S. Glutathione Transport: A New Role for PfCRT in Chloroquine Resistance. Antioxid Redox Signal. 2012 19(7):683-95 PMID:

See original on MMP

More information

PlasmoDB PF3D7_0512200
GeneDB PF3D7_0512200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL5P1.122, PFE0605c
Orthologs PBANKA_1111800 , PCHAS_1111400 , PKNH_1021400 , PVP01_1021800 , PVX_080630 , PY17X_1112900
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