Last updated 2 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
http://mpmp.huji.ac.il/maps/Merozoiteproteins.html Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA
Possible
PlasmoGEM (Barseq) Imported from PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) Imported from PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Localization of PfMA by Immuno-electron microscopy. Ultra thin sections of P. falciparum parasites at schizont/merozoite stages were labelled with mouse anti-PfMA or anti-EBA-175 antibodies followed with anti-mouse secondary antibody conjugated with 15nm colloidal gold particle. (A) Labelling with the PfMA antibody was confined only to the micronemes (arrows) in the apical end of the free merozoite and was absent from the rhoptry (Rh) or nucleus (Nu). (B, C) Similarly PfMA staining in the schizonts was observed only in the micronemes and not in the rhoptries. (D, E) A similar pattern of staining in the schizonts was observed with the micronemal marker EBA-175.Hans N, Singh S, Pandey AK, Reddy KS, Gaur D, Chauhan VS. Identification and Characterization of a Novel Plasmodium falciparum Adhesin Involved in Erythrocyte Invasion. PLoS One. 2013 Sep 13;8(9):e74790.

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PfMA localization in schizont stages analyzed by fluorescence microscopy. Sub-cellular localization of PfMA was studied by co-immunostaining with surface protein (A), microneme (B), rhoptry (C, D) resident proteins. P. falciparum schizonts were co-immunostained with mouse anti-PfMA (green) and rabbit antibodies against one of the 4 marker proteins (EBA175/PfRH2, ASP, MSP-119) (red). The nuclei of schizont were stained with DAPI (blue) and slides were visualized by fluorescence microscope. All apical marker proteins and PfMA showed punctate staining in schizonts. PfMA was localized in the micronemes as it signal costained with micronemal marker EBA-175. The scale bar indicates 2 μm.Hans N, Singh S, Pandey AK, Reddy KS, Gaur D, Chauhan VS. Identification and Characterization of a Novel Plasmodium falciparum Adhesin Involved in Erythrocyte Invasion. PLoS One. 2013 8(9):e74790.

See original on MMP

More information

PlasmoDB PF3D7_0316000
GeneDB PF3D7_0316000
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL3P6.1, PFC0700c
Orthologs PBANKA_0413800 , PCHAS_0414700 , PKNH_0826100 , PVP01_0824100 , PVX_095435 , PY17X_0416600
Google Scholar Search for all mentions of this gene