Last updated 1 year ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
10760158 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA
Possible
RMgm-140 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-141 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-4069 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
10760158 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Oocyst
Attenuated
10760158 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
No difference
RMgm-140 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-141 Imported from RMgmDB
P. yoelii yoelii 17X Asexual
No difference
RMgm-4069 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-140 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-141 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-4069 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-141
Mutants developed gametocytes and formed ookinetes in vitro in similar numbers to, and morphologically indistinguishable from, wild type parasites in Giemsa-stained preparations.Ookinetes do not develop into oocysts.After removal of the blood meal from dissected, gametocyte-infected Anopheles gambiae midguts, wild type ookinetes were found in the epithelium. In sharp contrast, no mutant ookinetes were observed. When dissected, infected A.stephensi midguts were examined for the presence of ookinetes or young oocysts on the basal surface, only wild type and never mutant parasites were observed. When ookinete motility was assessed by recording their positions on glass surfaces at 5 min time intervals, displacement of wildtype was observed but not mutant ookinetes, indicating that CTRP is involved in ookinete locomotion. The KO ookinetes were occasionally seen to straighten and bend.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-140
Normal numbers of ookinetes are produced (in vitro and in vivo in A. stephensi). Ookinetes do not show any morphological differences from wild-type parasites with Giemsa staining under microscopic observation. Ookinetes do not develop into oocysts.
Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
Difference from wild-type
RMgm-4069
Ookinetes show reduced motility (complete defect in forward gliding motility)
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-141
Normal numbers of ookinetes are produced. Ookinetes do not develop into oocysts.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-140
Normal numbers of ookinetes are produced. Ookinetes do not develop into oocysts.
Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
Difference from wild-type
RMgm-4069
Ookinetes show reduced motility (complete defect in forward gliding motility). No oocyst are formed.
Imported from RMgmDB

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