Last updated 3 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
35930605
Here we describe a protein PF3D7_0301700 (PTP7), that functions at the nexus between the intermediate trafficking organelle, the Maurer’s cleft, and the infected red blood cell surface. Genetic disruption of PTP7 leads to accumulation of vesicles at the Maurer’s clefts, grossly aberrant knob morphology, and failure to deliver EMP1 to the red blood cell surface. We show that an expanded low complexity sequence in the C-terminal region of PTP7, identified only in the Laverania clade of Plasmodium, is critical for efficient virulence protein trafficking.
Theo Sanderson, Francis Crick Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Altered cytoadherence
35930605
Here we describe a protein PF3D7_0301700 (PTP7), that functions at the nexus between the intermediate trafficking organelle, the Maurer’s cleft, and the infected red blood cell surface. Genetic disruption of PTP7 leads to accumulation of vesicles at the Maurer’s clefts, grossly aberrant knob morphology, and failure to deliver EMP1 to the red blood cell surface. We show that an expanded low complexity sequence in the C-terminal region of PTP7, identified only in the Laverania clade of Plasmodium, is critical for efficient virulence protein trafficking.
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

PFC0085c encodes an exported protein with a non-canonical PEXEL/HT. A. Fluorescence microscopy. The schematic structure of the GFP-tagged proteins is depicted on the left of representative images of iRBCs. B. Immunofluorescence analyses. Antibodies against GFP and the Maurer’s clefts protein REX2 were used. The ER-signal is shaded blue, the (non-) canonical PEXEL/HT is shaded black and GFP is shaded green. Putative cleavage sites are indicated by an arrow (blue: SP cleavage site; black: Plasmepsin V cleavage site. Fluorescence of GFP-tagged PFC0085c (03017-KSLAE-GFP), a protein with a K.L.E variant, indicated a Maurer’s clefts localization in addition to a uniformly distributed pool in the iRBC cytoplasm (A). This was confirmed by immunofluorescence analyses, demonstrating that 03017-KSLAE-GFP colocalized with REX2 (B).Schulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T, Pick C. The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins. Mol Microbiol. 2015 Apr 8. [Epub ahead of print]

See original on MMP

More information

PlasmoDB PF3D7_0301700
GeneDB PF3D7_0301700
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL3P8.15, PFC0085c
Orthologs
Google Scholar Search for all mentions of this gene