PFC0085c encodes an exported protein with a non-canonical PEXEL/HT. A. Fluorescence microscopy. The schematic structure of the GFP-tagged proteins is depicted on the left of representative images of iRBCs. B. Immunofluorescence analyses. Antibodies against GFP and the Maurer’s clefts protein REX2 were used. The ER-signal is shaded blue, the (non-) canonical PEXEL/HT is shaded black and GFP is shaded green. Putative cleavage sites are indicated by an arrow (blue: SP cleavage site; black: Plasmepsin V cleavage site. Fluorescence of GFP-tagged PFC0085c (03017-KSLAE-GFP), a protein with a K.L.E variant, indicated a Maurer’s clefts localization in addition to a uniformly distributed pool in the iRBC cytoplasm (A). This was confirmed by immunofluorescence analyses, demonstrating that 03017-KSLAE-GFP colocalized with REX2 (B).Schulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T, Pick C. The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins. Mol Microbiol. 2015 Apr 8. [Epub ahead of print]