Last updated 2 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
19073187 Theo Sanderson, Wellcome Trust Sanger Institute

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Upper panel: Double labelling of PfRON6 with AMA1 PF11_0344 in segmented schizonts. Confocal microscopy using anti-rPfRON6-B with anti-AMA1 (microneme marker). EM: Electron micrograph of immunogold staining using affinity purified anti-rRON6-C antibodies. Arrows indicate PfRON6.Right panel: Localisation of PfRON6 during the asexual red blood cell (RBC) cycle. Immunofluorescence microscopy using anti-rPfRON6-B antisera and DAPI on parasites at different stages of the asexual RBC cycle. The corresponding phase contrast images are shown.Proellocks NI, Kats LM, Sheffield DA, Hanssen E, Black CG, Waller KL, Coppel RL. Characterisation of PfRON6, a Plasmodium falciparum rhoptry neck protein with a novel cysteine-rich domain. Int J Parasitol. 2008 39(6):683-92. Copyright Elsevier 2009.

See original on MMP

Localisation of the PfRON6 protein in parasitised red blood cells. (B) Double labelling using anti-rPfRON6-B and anti- haemagglutinin (HA) in HA-tagged transgenic parasites. Confocal microscopy using anti-rPfRON6-B with anti-HA. Corresponding overlay images are shown. (C) Double labelling of PfRON6 with Pf34, PfRON4, RAMA or AMA1 in segmented schizonts. Confocal microscopy using anti-rPfRON6-B with anti-Pf34 (rhoptry neck marker), anti-PfRON4 (rhoptry neck marker), anti-RAMA (rhoptry bulb marker), or anti-AMA1 (microneme marker). Corresponding overlay images are shown. a punctate pattern of fluorescence in segmented schizonts characteristic of localisation with the apical secretory organelles.Proellocks NI, Kats LM, Sheffield DA, Hanssen E, Black CG, Waller KL, Coppel RL. Characterisation of PfRON6, a Plasmodium falciparum rhoptry neck protein with a novel cysteine-rich domain. Int J Parasitol. 2008 39(6):683-92. Copyright Elsevier 2009.

See original on MMP

More information

PlasmoDB PF3D7_0214900
GeneDB PF3D7_0214900
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PF02_0140, PFB0680w
Orthologs PBANKA_0311700 , PCHAS_0313800 , PKNH_0405900 , PVP01_0410000 , PVX_002790 , PY17X_0312200
Google Scholar Search for all mentions of this gene