Last updated 3 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
22127061 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
18083830
"a decrease in the rate of asexual growth in erythrocytes associated with a lower number of daughter merozoites generated per schizont, and second, a dramatic reduction in the ability to produce oocysts in the mosquito vector. A normal asexual growth rate and the ability to produce oocysts are restored if a functional copy of the PfPK7 gene is reintroduced into the PfPK7(-) parasites"
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
22348509
Do not respond to melatonin-stimulation, but do upon complementation.
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
RMgm-524 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
18083830
"a decrease in the rate of asexual growth in erythrocytes associated with a lower number of daughter merozoites generated per schizont, and second, a dramatic reduction in the ability to produce oocysts in the mosquito vector. A normal asexual growth rate and the ability to produce oocysts are restored if a functional copy of the PfPK7 gene is reintroduced into the PfPK7(-) parasites"
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Asexual
Difference from wild-type
22348509
Do not respond to melatonin-stimulation, but do upon complementation.
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Oocyst
Attenuated
18083830
"a decrease in the rate of asexual growth in erythrocytes associated with a lower number of daughter merozoites generated per schizont, and second, a dramatic reduction in the ability to produce oocysts in the mosquito vector. A normal asexual growth rate and the ability to produce oocysts are restored if a functional copy of the PfPK7 gene is reintroduced into the PfPK7(-) parasites"
Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
No difference
RMgm-524 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-524 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-524
Ookinete numbers (in vitro) reduced to 9% of wild type
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-524
Oocyst numbers (day 12-14) reduced to 9% of wild type. The remaining oocyst are growth arrested from about day 9
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-524
no sporozoites
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Immunofluorescence detection of PfPK7 expression during erythrocytic development. The IgY against a peptide derived from PfPK7 (see Experimental procedures) and a FITC-conjugated secondary antibody were used to investigate the localization of PfPK7 during erythrocytic development. The slides were pre-stained with DAPI to visualize nuclear DNA. Gam, gametocyte; Troph, trophozoite; Schiz, schizont. Immunofluorescence microscopy using the anti-peptide antibody shows that the PfPK7 protein localizes throughout the cytoplasm of both asexual parasites and gametocytes, with some punctuated pattern apparent in the latter, which has not yet been investigated further.Dorin D, Semblat JP, Poullet P, Alano P, Goldring JP, Whittle C, Patterson S, Chakrabarti D, Doerig C. PfPK7, an atypical MEK-related protein kinase, reflects the absence of classical three-component MAPK pathways in the human malaria parasite Plasmodium falciparum. Mol Microbiol. 2005 55:184-96.

See original on MMP

More information

PlasmoDB PF3D7_0213400
GeneDB PF3D7_0213400
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PF02_0125, PFB0605w
Orthologs PBANKA_0310300 , PCHAS_0312400 , PKNH_0407600 , PVP01_0411500 , PVX_002865 , PY17X_0310800
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