Last updated 2 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
30297725
\"Using CRISPR/Cas9 methodology we disrupted the gene encoding Pfp230p resulting in P. falciparum mutants (PfΔp230p) lacking P230p expression. The PfΔp230p mutants produced normal numbers of male and female gametocytes, which retained expression of P48/45 and P230. Upon activation male PfΔp230p gametocytes undergo exflagellation and form male gametes. However, male gametes are unable to attach to red blood cells resulting in the absence of characteristic exflagellation centres in vitro. In the absence of P230p, zygote formation as well as oocyst and sporozoite development were strongly reduced (>98%) in mosquitoes. These observations demonstrate that P230p, like P230 and P48/45, has a vital role in P. falciparum male fertility and zygote formation and warrants further investigation as a potential transmission blocking vaccine candidate.\"
Theo Sanderson, Francis Crick Institute
P. berghei ANKA
Possible
RMgm-351 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-352 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. knowlesi strain H
Possible
23267069 Theo Sanderson, Wellcome Trust Sanger Institute
P. yoelii yoelii 17X
Possible
RMgm-1099 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Gametocyte
Attenuated
30297725
\"Using CRISPR/Cas9 methodology we disrupted the gene encoding Pfp230p resulting in P. falciparum mutants (PfΔp230p) lacking P230p expression. The PfΔp230p mutants produced normal numbers of male and female gametocytes, which retained expression of P48/45 and P230. Upon activation male PfΔp230p gametocytes undergo exflagellation and form male gametes. However, male gametes are unable to attach to red blood cells resulting in the absence of characteristic exflagellation centres in vitro. In the absence of P230p, zygote formation as well as oocyst and sporozoite development were strongly reduced (>98%) in mosquitoes. These observations demonstrate that P230p, like P230 and P48/45, has a vital role in P. falciparum male fertility and zygote formation and warrants further investigation as a potential transmission blocking vaccine candidate.\"
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
No difference
RMgm-351 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-352 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. yoelii yoelii 17X Asexual
No difference
RMgm-1099 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-351 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-352 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-1099 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-351 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-352 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-1099 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-351 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-352 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-1099 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-351 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-352 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
No difference
RMgm-1099 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-351 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-352 Imported from RMgmDB

More information

PlasmoDB PF3D7_0208900
GeneDB PF3D7_0208900
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PF02_0085, PFB0400w
Orthologs PBANKA_0306000 , PCHAS_0308200 , PKNH_0412200 , PVP01_0415900 , PVX_003900 , PY17X_0306600
Google Scholar Search for all mentions of this gene