Last updated 2 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
23729665 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
RMgm-1500 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1372 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-1098 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-1500 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1372 Imported from RMgmDB
P. yoelii yoelii 17X Asexual
No difference
RMgm-1098 Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-1500
Male and female gametes fail to egress from their host erythrocytes after induction of gametogenesis.
Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-1372
Strongly reduced exflagellation
Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-1098 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-1372
Strongly reduced ookinete numbers
Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-1098 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1500
No oocyst formation
Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
Difference from wild-type
RMgm-1098
No (mature) oocysts are formed
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1500
No oocyst and sporozoite formation
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1372
No sporozoites in vivo
Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
Difference from wild-type
RMgm-1098
No (mature) oocysts are formed. No sporozoites are formed
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1372
No transmission by bite of infected mosquitoes
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

PfPAT localization in P. falciparum-infected erythrocytes. (A) localization of PfPAT-GFP by imunofluorescence analysis using anti-GFP (green). The red blood cell membrane marker Band3 (red) was detected using an anti-Band3 monoclonal antibody. Parasite nucleus was visualized using the Hoescht 33258 dye (blue). PfPAT signal is seen both on the parasite plasma membrane as well as associated with vesicular structures extending from the nuclear membrane in all blood stages – probably due to over expression. (B) Transmission electron micrograph of ultrathin cryosections of the intraerythrocytic early trophozoite stage of P. falciparum PfPAT-GFP transgenic parasites using anti-GFP antibody (18-nm gold particles; indicated with arrowheads). PPM: parasite plasma membrane. RBC: red blood cell. RBCM: red blood cell membrane. Most PfPAT signal (~68% of gold particles) is found to be associated with the parasite plasma membrane. (D) Localization of PfPAT in P. falciparum 3D7 parasites at the schizont stage using anti-PfPAT antibodies (green). The red blood cell membrane marker Band3 (red) was detected using an anti-Band3 monoclonal antibody. Parasite nucleus was visualized using the Hoescht 33258 dye (blue). Localization of the protein to the plasma membrane of the parasite is seen at all stages of the parasite intraerythrocytic life cycle. This localization is particularly noticeable in parasites at the schizont stage with fluorescence signal surrounding each individual merozoite.Augagneur Y, Jaubert L, Schiovani M, Pachikara N, Garg A, Usmani-Brown S, Wesolowski D, Zeller S, Ghosal A, Cornillot E, Said HM, Kumar P, Altman S, Ben Mamoun C. Identification and Functional Analysis of the Primary Pantothenate Transporter, PfPAT, of the Human Malaria Parasite Plasmodium falciparum. J Biol Chem. 2013 288(28):20558-67.

See original on MMP

More information

PlasmoDB PF3D7_0206200
GeneDB PF3D7_0206200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PF02_0058, PFB0275w
Orthologs PBANKA_0303900 , PCHAS_0306100 , PKNH_0414700 , PVP01_0418900 , PVX_003745 , PY17X_0304500
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