Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Localisation of PHIST:GFP proteins. The left- and right-hand images show GFP localisation and a phase contrast image, respectively. White filled arrows: peripheral GFP puncta; White unfilled arrow: GFP puncta in host erythocyte cytosol. Scale bar, 2 mm. GFP-tagged PF3D7_0401800, PF3D7_0424600, PF3D7_0532400, PF3D7_1102500 and PF3D7_1476200 were all exported to the host cell and displayed a striking localisation at the edge of the host erythrocyte (1A–E), PF3D7 1252700 was clearly peripheral in the host erythrocyte (F). PF3D7_0201600 (G) shows weak accumulation at the erythrocyte periphery, but the majority of the protein was localised in the RBC cytosol. PF3D7_0936600 was localised in the erythrocyte cytosol, not at the host cell periphery (H).Tarr SJ, Moon RW, Hardege I, Osborne AR. A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes. Mol Biochem Parasitol. 2014 196(1):29-40 PMID: 25106850

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PFB0080c is expressed on the IRBC surface of C4S-selected P. falciparum. A-D, Immunofluorescence analysis of IRBCs was performed using anti-PFB0080c antiserum followed by either FITC-conjugated goat anti-mouse IgG (A-C) or phycoerythrin-conjugated goat antimouse IgG (D) secondary antibody. A and B, Permeabilized C4S-adherent (3D7-CSA) IRBCs (A) and non-selected (3D7-NS) IRBCs (B). C and D, Live 3D7-CSA IRBCs. PFB0080c is strongly expressed on the surface of C4S adherent IRBCs, but not in 3D7-NS IRBCs.Goel S, Muthusamy A, Miao J, Cui L, Salanti A, Winzeler EA, Gowda DC. Targeted disruption of a ring-infected erythrocyte surface antigen (RESA)-like export protein gene in Plasmodium falciparum confers stable chondroitin 4-sulfate cytodherence capacity. J Biol Chem. 2014 Oct 23. [Epub ahead of print]

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PFB0080c-knockout P. falciparum express VAR2CSA on the surface of infected erythrocytes. A and B, Immuno-fluorescence analysis of VAR2CSA in live ΔPFB0080c IRBCs was performed using rabbit anti-VAR2CSA antiserum followed by biotin-conjugated sheep antirabbit IgG secondary antibody and phycoerythrin-conjugated streptavidin. Shown are the immunofluorescent and light micrographs of parental 3D7-CSA parasites (A) and ΔPFB0080c parasites (B); both parasite types expressed VAR2CSA protein. Goel S, Muthusamy A, Miao J, Cui L, Salanti A, Winzeler EA, Gowda DC. Targeted disruption of a ring-infected erythrocyte surface antigen (RESA)-like export protein gene in Plasmodium falciparum confers stable chondroitin 4-sulfate cytodherence capacity. J Biol Chem. 2014 Oct 23. [Epub ahead of print]

See original on MMP

Localisation of PHIST:GFP proteins. The left- and right-hand images show GFP localisation and a phase contrast image, respectively. White filled arrows: peripheral GFP puncta; White unfilled arrow: GFP puncta in host erythocyte cytosol. Scale bar, 2 mm. GFP-tagged PF3D7_0401800, PF3D7_0424600, PF3D7_0532400, PF3D7_1102500 and PF3D7_1476200 were all exported to the host cell and displayed a striking localisation at the edge of the host erythrocyte (1A–E), PF3D7 1252700 was clearly peripheral in the host erythrocyte (F). PF3D7_0201600 (G) shows weak accumulation at the erythrocyte periphery, but the majority of the protein was localised in the RBC cytosol. PF3D7_0936600 was localised in the erythrocyte cytosol, not at the host cell periphery (H).Tarr SJ, Moon RW, Hardege I, Osborne AR. A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes. Mol Biochem Parasitol. 2014 196(1):29-40

See original on MMP

More information

PlasmoDB PF3D7_0201600
GeneDB PF3D7_0201600
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PF02_0016, PFB0080c
Orthologs
Google Scholar Search for all mentions of this gene