Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-385 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-385 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-385 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-385 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-385
Fluorescence microscopy revealed that c-cap(-) oocysts were markedly reduced in size and numbers as compared to the wild tye. c-cap(-) parasites consistently formed slightly fewer oocysts, i.e. on average 110 ( 42) oocysts per infected mosquito as compared to an average of 213 ( 64) oocysts per infected mosquito for wild type. Up until day 6 oocyst development was comparable between c-cap(-) and wildtype, indicating that initial events in sporogony, including increase of parasite mass and the first few mitotic replications, were unaffected in the mutant. By day 8 c-cap(-) oocysts were significantly smaller in size and arrested their development, when compared to the linear size increase in wildtype oocysts. Quantification of oocyst diameters revealed that c-cap(-) occysts typically reached an average size of 15 m, corresponding to day 8 wild type oocysts. However, few c-cap(-) oocysts reached sizes comparable to wild type. In mutant oocysts only few nuclei corresponding to 4-5 rounds of nuclear divisions and no sporozoites were detected. No formation of sporozoites inside the oocysts.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-385
No formation of sporozoites within the oocyst. No salivary gland sporozoites
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-385
No formation of sporozoites within the oocyst. No salivary gland sporozoites. Infected mosquitoes are not able to infect mice.
Imported from RMgmDB

More information

PlasmoDB PF3D7_0105300
GeneDB PF3D7_0105300
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL1P1.43, PFA0260c
Orthologs PBANKA_0208000 , PCHAS_0206400 , PKNH_0208400 , PVP01_0209500 , PVX_081498 , PVX_081502 , PY17X_0209400
Google Scholar Search for all mentions of this gene