Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-139 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-138 Imported from RMgmDB
P. falciparum 3D7
Possible
28355563
No cell traversal
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-139 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-138 Imported from RMgmDB
P. falciparum 3D7 Asexual
No difference
28355563
No cell traversal
Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Gametocyte
No difference
RMgm-139 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-138 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-139 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-138 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-139 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-138 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-139
Normal numbers of midgut- and salivary gland sporozoites are formed. Sporozoites showed wild type gliding motility. Sporozoites had lost cell passage ability (membrane-damaging capacity) as shown in the cell-wounding assay. Compared to wild type sporozoites, the mutant sporozoites were rapidly immobilized in the dermis. Mutant sporozoites were shown to be arrested by and invade dermal fibroblasts.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-138
Normal numbers of midgut- and salivary gland sporozoites are formed. Sporozoites showed normal gliding mitility. Sporozoites have a strongly reduced infectivity (15-28 fold lower) to rats after intravenous injection of sprozoites and strongly reduced numbers of exoerythrocytic forms (EEFs) in frozen sections of rat livers (30-fold decrease) were counted 24 h after sporozoite inoculations.
Imported from RMgmDB
P. falciparum 3D7 Sporozoite
Attenuated
28355563
No cell traversal
Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Liver
Difference from wild-type
RMgm-139
No difference was noticed between wild type and mutant parasites in number, size, and fluorescence intensity of EEF at 4, 12, 24, or 48 hr in rat or mouse primary hepatocytes. Sporozoites show in vitro a 'rapid invader' phenotype, as a result of the lack of cell traversal. Mutant sporozoites show an adhesion/attachment phenotype (to CRL-2017 cells in vitro) which is comparable to wild type sporozoites.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-138
Sporozoites have a strongly reduced infectivity (15-28 fold lower) to rats after intravenous injection of sprozoites and strongly reduced numbers of exoerythrocytic forms (EEFs) in frozen sections of rat livers (30-fold decrease) were counted 24 h after sporozoite inoculations. Sporozoites formed normal numbers of EEFs in hepatoma cells (HepG2), indicating that they retain normal infection ability. Sporozoites had completely lost cell passage ability as shown in the cell-wounding assay. Cell-passage activity was estimated by the number of cells wounded by sporozoite passage, which were identified by cytosolic labelling with FITC-conjugated dextran.In Kupffer cell-depleted rats, the sporozoites had restored liver infectivity. Parasitaemia after inoculation of mutant sporozoites was remarkably increased in Kupffer-depleted rats, becoming similar to that after inoculation of wild-type sporozoites In addition, the numbers of EEFs formed in the liver were also similar between spect-disruptants and the wild-type.
Imported from RMgmDB

More information

PlasmoDB PCHAS_1360200
GeneDB PCHAS_1360200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PCHAS_136020
Orthologs PBANKA_1355600 , PF3D7_1342500 , PKNH_1258900 , PVP01_1212300 , PVX_083025 , PY17X_1361300
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