Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
24583112 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Indirect immunofluorescence assay of P. falciparum acetyl CoA carboxylase-haemagluttinin transgenic parasites showing localisation of P. falciparum acetyl CoA carboxylase in the apicoplast (green, P. falciparum acetyl CoA carboxylase-haemagluttinin labelled with anti-haemagluttinin antibody; red, apicoplast labelled with anti-Pf acyl carrier protein; blue, DNA stained with Hoechst 33342). Bar = 1 mm. The PfACC gene thus encodes an apicoplast-localised protein.Goodman CD, Mollard V, Louie T, Holloway GA, Watson KG, McFadden GI. Apicoplast acetyl Co-A carboxylase of the human malaria parasite is not targeted by cyclohexanedione herbicides. Int J Parasitol. 2014 Feb 25. pii: S0020-7519(14)00034-4. PMID:

See original on MMP

PfAMA1 translocation is aberrant in schizonts with PfMOP-knockdown. (a) Schizonts from [+]/[-] Shld1 PfMOP-DD parasites were E64-treated, fixed, probed with anti-PfAMA1 and scored as micronemal (M), partially translocated (PT) or surface (S), representative [+] Shld1 parasite IFAs shown. (c) Synchronized schizont stage (40–44 h) parasites, maintained with 250nM (left panel) or 0 nM (right panel) Shld1, were incubated 6 h in presence of 10 mM E64, methanol-fixed, permeabilized, and stained using antibodies against PfRON4, PfRhopH3, PfEBA175 and PfTubulin. Staining for these markers was similar in [+] and [-] Shld1 conditions. PfAMA1 staining was used to identify E64-treated schizonts that were sufficiently mature (that is, surface staining or partially translocated staining, but not micronemal staining, scale bar, 1 mm).Absalon S, Robbins JA, Dvorin JD. An essential malaria protein defines the architecture of blood-stage and transmission-stage parasites. Nat Commun. 2016 7:11449.

See original on MMP

More information

PlasmoDB PCHAS_1337400
GeneDB PCHAS_1337400
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PC000014.02.0, PC000605.02.0, PC103333.00.0, PC300459.00.0, PC300489.00.0, PC301889.00.0, PC302471.00.0, PCAS_133740, PCHAS_133740
Orthologs PBANKA_1332800 , PF3D7_1469600 , PKNH_1211400 , PVP01_1237200 , PVX_116985 , PY17X_1337500
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