Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-816 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-4361 Imported from RMgmDB
P. falciparum 3D7
Possible
15225318 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Difference from wild-type
RMgm-816
Reduced growth rate of blood stages (normal production of hemozoin)
Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Asexual
Difference from wild-type
RMgm-4361
(Strongly) reduced growth rate of blood stages
Imported from RMgmDB
P. falciparum 3D7 Asexual
No difference
15225318 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Oocyst
No difference
RMgm-4361 Imported from RMgmDB
P. falciparum 3D7 Oocyst
Attenuated
15225318 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Sporozoite
No difference
RMgm-4361 Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-4361
Prolonged prepatent period (4 days delay) after infection of mice with sporozoites (see additional information). Normal hepatocyte invasion and development of liver stages. Normal merosome formation and rupture. Evidence is presented for reduced hepatic merozoite infectivity.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Falcipain 1 and EBA-175 (a merozoite microneme protein) co-localized at the schizont stage. Immunofluorescence images of fixed 3D7 P. falciparum parasites. (A) Merozoites costained with anti-F1.4 and anti-EBA-175 antibodies. (B) Schizonts costained with anti-F1.4 and anti-EBA-175 antibodies. (C) Merozoites costained with anti-F1.4 and anti-MSP-119 antibodies.Kumar A, Kumar K, Korde R, Puri SK, Malhotra P, Singh Chauhan V. Falcipain-1, a Plasmodium falciparum cysteine protease with vaccine potential. Infect Immun. 2007 75:2026-34.

See original on MMP

Immunoelectron microscopy of P. falciparum infected trophozoites labelled with Ab574 against falcipain I. In all micrographs 'e' signifies erythrocyte, 'cā€˜ signifies cytostome, and bars = 0.5mm. (A) A general view of the cell showing label associated with a hemoglobin transport vacuole (closed arrow), the surface of the parasite (open arrow), and the condensed hemozoin in the food vacuole. (B) A micrograph showing label in the vesicular omponents that subtend the surface membrane complex of the parasite. (C) A micrograph demonstrating the labeling of the pellicular membranes. (D) A section through a cytostome of the parasite (open arrow). Membrane processes are present in the lumen of the cytostome. (E) The hemoglobin transport vesicles label strongly with antibody, predominantly in the lumen of the vesicle. (F) The antibody also reacts with the digestive vacuole itself, where it is associated with the hemozoin.Francis SE, Gluzman IY, Oksman A, Knickerbocker A, Mueller R, Bryant ML, Sherman DR, Russell DG, Goldberg DE. Molecular characterization and inhibition of a Plasmodium falciparum aspartic hemoglobinase. EMBO J. 1994 Jan 15;13(2):306-17.PubMed PMID:

See original on MMP

Localization of falcipain 1 (FP1) in merozoites by immunofluorescence imaging. Samples of merozoites were smeared and fixed in 1% paraformaldehyde. The slides were incubated with an antibody to falcipain 1 or with antibodies specific to the rhoptry (RopH2) and microneme (EBA-175) proteins. Nuclei were stained with 4ā€™,6-diamidino-2-phenylindole (DAPI). The lack of colocalization indicates that falcipain 1 is found in compartments at the apical end of the merozoite that are distinct from the rhoptries and micronemes.Greenbaum DC, Baruch A, Grainger M, Bozdech Z, Medzihradszky KF, Engel J, DeRisi J, Holder AA, Bogyo M. A role for the protease falcipain 1 in host cell invasion by the human malaria parasite. Science. 2002 298(5600):2002-6.

See original on MMP

More information

PlasmoDB PCHAS_1325000
GeneDB PCHAS_1325000
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PC000637.04.0, PCAS_132500, PCHAS_132500
Orthologs PBANKA_1321700 , PF3D7_1458000 , PKNH_1223800 , PVP01_1248900 , PVX_117565 , PY17X_1325500
Google Scholar Search for all mentions of this gene