Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-4065 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1299 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA
Possible
RMgm-4824 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-4825 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-4065 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Asexual
No difference
RMgm-4824 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-4825 Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-4065
Normal gametocyte numbers and sex ratio. Mean male gamete exflagellation events were slightly but significantly reduced in the psop25 line, as compared to the WT parasites.
Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-4824 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-4825 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-4065
Significantly reduced ookinete production (60% in vitro). Mature ookinetes formed showed normal (light microscope) morphology.
Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1299 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-4824 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-4825 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4065
Significantly reduced oocyst production (69%)
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1299
More than 20-fold reduction in oocyst numbers.The size of parasite oocysts was clearly smaller than that of the wild-type parasite oocysts [as observed by phase contrast microscopy at 14 days post-infection (dpi). To identify the step in which they decreased in number, parasites were generated that constitutively expressed GFP from these disruptant populations and the oocysts shortly after ookinete invasion of the midgut at 2 dpi were counted. The number of oocysts was already approximately 10-fold smaller than that of wild-type parasites.We further measured oocyst diameters by epifluorescence microscopy at 14 dpi. The average oocyst diameter was approximately 60% of that of the wild-type oocysts, suggesting that oocyst development was impaired by this disruption.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4824
In Anopheles coluzzi no oocyst formation
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4825
In Anopheles coluzzi no oocyst formation
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1299
Strongly reduced sporozoite formation
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-4824
In Anopheles coluzzi no oocyst formation; no sporozoite formation (in A stephensi low numbers of oocysts were observed, but no sporozoites).
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-4825
In Anopheles coluzzi no oocyst formation; no sporozoite formation (in A stephensi low numbers of oocysts were observed, but no sporozoites).
Imported from RMgmDB

More information

PlasmoDB PCHAS_1118700
GeneDB PCHAS_1118700
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PC000012.05.0, PCAS_111870, PCHAS_111870
Orthologs PBANKA_1119200 , PF3D7_0620000 , PKNH_1130300 , PKNH_1130900 , PVP01_1129500 , PVX_114125 , PY17X_1120300
Google Scholar Search for all mentions of this gene