Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

PfKMT1 and genes enriched in H3K9me3 are positioned at the nuclear periphery. Immuno-fluorescence assays using antibodies against H3K9me3 H3 (right) and the P. falciparum putative H3K9 methyltransferase PfKMT1 (left). 3D7 parasites are in late schizont (main image) and ring stage (top-right square). Nuclear DNA was detected with DAPI (red).Lopez-Rubio JJ, Mancio-Silva L, Scherf A. Genome-wide analysis of heterochromatin associates clonally variant gene regulation with perinuclear repressive centers in malaria parasites. Cell Host Microbe. 2009 5:179-90.

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SET-domain proteins PF08_0012 and Histone-lysine N-methyltransferase PF13_0293 localize to the nuclear periphery marked by CenPA. (A) Localization of PF08_0012 reveals a clustered localization pattern present throughout the nuclear periphery of the cell. PF08_0012 is HA-tagged at the C-terminus and detected with anti-HA antibodies. (B) Live imaging of PF13_0293 shows localization to a distinct area in the nuclear periphery. Co-localization studies by immunofluorescence are shown. Nup100 (PFI0250c) and CenPA (histone H3 PF13_0185) are used as markers of the nuclear periphery.Volz J, Carvalho TG, Ralph SA, Gilson P, Thompson J, Tonkin CJ, Langer C, Crabb BS, Cowman AF. Potential epigenetic regulatory proteins localise to distinct nuclear sub-compartments in Plasmodium falciparum. Int J Parasitol. 2010 40:109-21. Copyright Elsevier 2010.

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Schematic representation of the Plasmodium falciparum nuclear architecture. A schematic representation of an infected red blood cell (RBC) and an enlargement of the P. falciparum nucleus are shown. From the centre towards the periphery we have defined three distinct areas (1–3) in which nuclear proteins specifically distribute. A summary is shown in which trophozoite stages have been analyzed. Proteins, previously predicted to functionally interact, are shown in red. Proteins previously predicted for their histone target sites are indicated by grey boxes. A, apicoplast. M, mitochondria, No, nucleolus.Volz J, Carvalho TG, Ralph SA, Gilson P, Thompson J, Tonkin CJ, Langer C, Crabb BS, Cowman AF. Potential epigenetic regulatory proteins localize to distinct nuclear sub-compartments in Plasmodium falciparum. Int J Parasitol. 2010 40(1):109-21. Copyright Elsevier.

See original on MMP

More information

PlasmoDB PCHAS_0935100
GeneDB PCHAS_0935100
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PC000278.00.0, PC000615.01.0, PCAS_093510, PCHAS_093510
Orthologs PBANKA_0702900 , PF3D7_0827800 , PKNH_1321900 , PVP01_0506600 , PVX_088965 , PY17X_0703200
Google Scholar Search for all mentions of this gene