Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Cell cycle arrest
25043062 (Conditional)
drastic morphological changes in mature stages of the second cycle and were deficient in progressing to a third cycle
Mehdi, Sanger

Imaging data (from Malaria Metabolic Pathways)

Coimmunofluorescence assay of three plasmodial exoribonucleases and a core exosome member, PfRrp45, in ring-stage wild-type 3D7 parasites. The rabbit anti-PfRrp45, anti-PfRNase II, anti-PfRrp6 and mouse anti-PfDis3 antibodies were used in these assays. DAPI, 49,6-diamidino-2-phenylindole. Scale bar, 1 mm. Immunofluorescence assay (IFA) analysis revealed that PfRrp6 and PfRNase II localize as distinct foci at the nuclear periphery and that PfDis3 is localized in the cytoplasm adjacent to the nucleus at the ring stage .Zhang Q, Siegel TN, Martins RM, Wang F, Cao J, Gao Q, Cheng X, Jiang L, Hon CC, Scheidig-Benatar C, Sakamoto H, Turner L, Jensen AT, Claes A, Guizetti J, Malmquist NA, Scherf A. Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria. Nature. 2014 Jun 29. PMID:

See original on MMP

More information

PlasmoDB PCHAS_0416200
GeneDB PCHAS_0416200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PC000442.02.0, PCAS_041620, PCHAS_041620
Orthologs PBANKA_0415300 , PF3D7_0906000 , PKNH_0703800 , PVP01_0704500 , PVX_098745 , PY17X_0418100
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