Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
RMgm-994 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Subcellular localization of SufE to the apicoplast. Upper panel) Epifluorescent images of live P. falciparum erythrocytic-stage parasites expressing GFP fused to full length SufE (SufEfl-GFP). The parasites were stained with mitotracker to identify mitochondria and DAPI to identify nuclei. Image z-stacks were deconvolved and then presented as a single combined image. Scale bar = 2 mm. GFP fluorescence localizes to an elongated organelle distinct from the mitochondrion in late ring (top row), late trophozoite or early schizont (middle), and schizont (bottom) stage parasites. Lower panel) Immuno-fluorescence co-localization of SufEfl-GFP and endogenous ACP. An antibody specific for GFP co-localized with αACP antibodies, demonstrating apicoplast localization in late ring (top row), late trophozoite or early schizont (middle), and schizont (bottom) stage parasites. The parasites were stained with DAPI to identify nuclei. Image z-stacks were deconvolved and then presented as a single combined image. Scale bar = 2 µm.Gisselberg JE, Dellibovi-Ragheb TA, Matthews KA, Bosch G, Prigge ST. The Suf Iron-Sulfur Cluster Synthesis Pathway Is Required for Apicoplast Maintenance in Malaria Parasites. PLoS Pathog. 2013 9(9):e1003655 Ì

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Immunofluorescence localisation of PfSufS and PfSufE. (A) Immunofluorescence assay of P. falciparum 3D7 cells using anti-PfSufS Ab with anti-PfHU Ab as apicoplast marker (panel i) or Mitotracker Red (panel ii) indicates targeting of PfSufS to the apicoplast. (B) Co-localisation of PfSufE and apicoplast-targeted GFP observed in the P. falciparum D10 ACPleader-GFP cell line using anti-PfSufE and anti-GFP antibodies (panel i). No overlap of PfSufE signal was observed with Mitotracker Red in P. falciparum 3D7 cells (panel ii) indicating apicoplast localization of PfSufE. PfSufS co-localised with PfHU, an apicoplast DNA condensation protein and no overlapping signals for PfSufS were seen with the mitochondrial marker Mitotracker Red in P. falciparum 3D7 cells (A). PfSufE was localised in the P. falciparum D10-ACPleader-GFP line that targets GFP to the apicoplast. Specific PfSufE signals overlapping with apicoplast-targeted GFP were observed and no overlap of PfSufE signal was seen with Mitotracker Red (B). The localisation of PfSufS and PfSufE exclusively to the apicoplast was thus confirmed.Charan M, Singh N, Kumar B, Srivastava K, Siddiqi MI, Habib S. Sulphur mobilisation for [Fe-S] cluster assembly by the essential SUF pathway in the Plasmodium falciparum apicoplast and its inhibition. Antimicrob Agents Chemother. 2014 Apr 7. [Epub ahead of print]

See original on MMP

Immunofluorescence localisation of PfSufS and PfSufE. (A) Immunofluorescence assay of P. falciparum 3D7 cells using anti-PfSufS Ab with anti-PfHU Ab as apicoplast marker (panel i) or Mitotracker Red (panel ii) indicates targeting of PfSufS to the apicoplast. (B) Co-localisation of PfSufE and apicoplast-targeted GFP observed in the P. falciparum D10 ACPleader-GFP cell line using anti-PfSufE and anti-GFP antibodies (panel i). No overlap of PfSufE signal was observed with Mitotracker Red in P. falciparum 3D7 cells (panel ii) indicating apicoplast localization of PfSufE. PfSufS co-localised with PfHU, an apicoplast DNA condensation protein and no overlapping signals for PfSufS were seen with the mitochondrial marker Mitotracker Red in P. falciparum 3D7 cells (A). PfSufE was localised in the P. falciparum D10-ACPleader-GFP line that targets GFP to the apicoplast. Specific PfSufE signals overlapping with apicoplast-targeted GFP were observed and no overlap of PfSufE signal was seen with Mitotracker Red (B). The localisation of PfSufS and PfSufE exclusively to the apicoplast was thus confirmed.Charan M, Singh N, Kumar B, Srivastava K, Siddiqi MI, Habib S. Sulphur mobilisation for [Fe-S] cluster assembly by the essential SUF pathway in the Plasmodium falciparum apicoplast and its inhibition. Antimicrob Agents Chemother. 2014 Apr 7. [Epub ahead of print

See original on MMP

More information

PlasmoDB PCHAS_0306000
GeneDB PCHAS_0306000
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PC000258.03.0, PC000609.00.0, PC108508.00.0, PCAS_030600, PCHAS_030600
Orthologs PBANKA_0303800 , PF3D7_0206100 , PKNH_0414800 , PVP01_0419000 , PVX_003740 , PY17X_0304400
Google Scholar Search for all mentions of this gene