Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-121 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-118 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-121 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-118 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
No difference
RMgm-121 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-118 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-121 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-118 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-121
Normal numbers of oocysts are produced. Sporozoite formation within the oocysts is blocked. Analysis of the morphology of oocysts by light microscopy revealed that the majority of 1722 day oocysts were either vacuolated or non-sporulated, and did not contain sporozoites.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-118
Normal numbers of oocysts are produced. Sporozoite formation within the oocysts is blocked. The diameters of oocysts were significantly larger than that of wild type on days 14 and 21 of infection. Light microscopy revealed the presence of two distinct populations of oocysts: those that displayed a phenotype reminiscent of immature wild type oocysts (i.e., non-sporulated), and those that appeared vacuolated/degenerate compared to wild type. Transmission electron microscopy analysis of oocysts further confirmed these findings and revealed that oocysts of these parasites possessed an endoplasmic reticulum that was highly vacuolated compared to that of wild type parasites. On day 13 p.i., the nuclear organization of oocysts appeared immature as indicated by the presence of few but large nuclei. By comparison, wild type oocysts of the same age had formed sporozoites, each with their own (haploid) nucleus.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-121
No hemocoel and salivary gland sporozoites were detected.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-118
No midgut sporozoites were observed on day 10/11 p.i. By day 18 p.i., reduced numbers (typically 0%12%) of sporozoites were observed in dissected midguts. The number of sporozoites in salivary gland preparations infections was consistently reduced to <1% of wild type. The expression and targeting of the circumsporozoite protein in midgut sporozoites was indistinguishable from that in wild type.To test if the observed sporozoites were infectious to mice, infected mosquitoes were allowed to feed on mice on days 21 and 28 p.i. Blood stage parasites were observed in all mice bitten by wild type-infected mosquitoes when screened on day 4/5 post-bite. In contrast, mice bitten by mutant-infected mosquitoes remained uninfected.
Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-118 Imported from RMgmDB

More information

PlasmoDB PBANKA_1300700
GeneDB PBANKA_1300700
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) LAP2, PB000652.01.0, PB001034.03.0, PB300439.00.0, PBANKA_130070
Orthologs PCHAS_1303900 , PF3D7_1475500 , PKNH_1245800 , PVP01_1270400 , PVX_118650 , PY17X_1304300
Google Scholar Search for all mentions of this gene