Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-1467 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1466 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Difference from wild-type
RMgm-1467
While no phenotypic differences were observed between the gene-deletion mutants and wild-type (wt) parasites during development in the mosquito or in the liver, blood stages of the mutants exhibited an attenuated phenotype in mice. While wt-infected C57B/6 mice died 79 days post infection as a result of complications typical for experimental cerebral malaria, Pbmahrp1a-infected mice did not develop experimental cerebral malaria but died from hyperparasitemia 3245 days post infection, respectively. A similar attenuation was also observed in infections in Balb/c mice. The parasitemia in the Pbmahrp1a-infected mice was comparable to that of wt during the initial 2 days of infection, but was reduced compared with wt thereafter. Mutant parasites were predominantly found in reticulocytes throughout the course of infection, whereas virulent wt parasites were found in erythrocytes in the later course of infection.In addition, mice infected with thePbmahrp1a mutant showed a 3.6-fold increase in schizonts in the peripheral blood circulation compared to wt infections, respectively , indicating reduced sequestration of RBCs infected with mutant schizonts.Pbmahrp1a parasites showed strongly reduced accumulation in the adipose tissue and lungs compared with wt, which is typical for a loss of CD36-mediated sequestration. Conversely, significantly increased accumulation of parasites was detected in the spleens of mice infected with Pbmahrp1a mutants. Higher accumulation in the spleen of non-sequestered schizonts has been attributed to increased removal of non-sequestering schizonts by the spleen.Binding to mouse CD36 was significantly lower in Pbmahrp1a schizonts compared with wt schizonts with both plate-coated CD36 or CD36 expressed on CHO-745 cells.
Imported from RMgmDB
P. berghei ANKA Asexual
Difference from wild-type
RMgm-1466
While no phenotypic differences were observed between the gene-deletion mutants and wild-type (wt) parasites during development in the mosquito or in the liver, blood stages of the mutants exhibited an attenuated phenotype in mice. While wt-infected C57B/6 mice died 79 days post infection as a result of complications typical for experimental cerebral malaria, Pbmahrp1a-infected mice did not develop experimental cerebral malaria but died from hyperparasitemia 3245 days post infection, respectively. A similar attenuation was also observed in infections in Balb/c mice. The parasitemia in the Pbmahrp1a-infected mice was comparable to that of wt during the initial 2 days of infection, but was reduced compared with wt thereafter. Mutant parasites were predominantly found in reticulocytes throughout the course of infection, whereas virulent wt parasites were found in erythrocytes in the later course of infection.In addition, mice infected with thePbmahrp1a mutant showed a 3.6-fold increase in schizonts in the peripheral blood circulation compared to wt infections, respectively , indicating reduced sequestration of RBCs infected with mutant schizonts.Pbmahrp1a parasites showed strongly reduced accumulation in the adipose tissue and lungs compared with wt, which is typical for a loss of CD36-mediated sequestration. Conversely, significantly increased accumulation of parasites was detected in the spleens of mice infected with Pbmahrp1a mutants. Higher accumulation in the spleen of non-sequestered schizonts has been attributed to increased removal of non-sequestering schizonts by the spleen.Binding to mouse CD36 was significantly lower in Pbmahrp1a schizonts compared with wt schizonts with both plate-coated CD36 or CD36 expressed on CHO-745 cells.
Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-1467 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-1466 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-1467 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-1466 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-1467 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-1466 Imported from RMgmDB

More information

PlasmoDB PBANKA_1145800
GeneDB PBANKA_1145800
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PB102173.00.0, PB400017.00.0, PBANKA_114580
Orthologs PCHAS_1145300 , PY17X_1147600
Google Scholar Search for all mentions of this gene