Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
19164769 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
19164769 (Conditional) Theo Sanderson, Wellcome Trust Sanger Institute

Imaging data (from Malaria Metabolic Pathways)

Confocal microscopic findings of Pf-calpain of P. falciparum in the erythrocytic cycle. Panel A shows P. falciparum revealed by Giemsa staining. Panels B–E show immunofluorescence results. (B) The blue color is nuclear staining by DAPI. (C) The red color is Pf-calpain staining by anti-Pf-calpain antibody A (panels B and C) and antibody B (panels D and E) and Cy-3-conjugated secondary antibodies. Panels B and D show the immunofluorescence in darkness and panels C and E show the immunofluorescence in bright light as a merged image. ×1000 magnification.Choi YY, Jung SY, Soh BY, Zheng B, Kim S, Park KI, Park H. Confocal microscopic findings of cysteine protease calpain in Plasmodium falciparum. Exp Parasitol. 2009 24:341-345. Copyright Elsevier 2010.

See original on MMP

Localization of Pf-Hsp60 and Pf-calpain in the infected RBCs. Co-localization of Pf-Hsp60 and Pf-calpain was observed by IFA. Anti-GroEL-antibody was used for detection of Pf-Hsp60 using FITC-conjugated rabbit-anti-mouse IgG (green). Pf-calpain was visualized by Cy5-conjugated goat-anti-rabbit IgG (red). Pf-Hsp60 was partially co-localized (yellow) with Pf-calpan in both trophozoites (A) and schizonts (B).Yeo SJ, Liu DX, Park H. Potential Interaction of Plasmodium falciparum Hsp60 and Calpain. Korean J Parasitol. 2015 Dec;53(6):665-73.

See original on MMP

More information

PlasmoDB PBANKA_1138400
GeneDB PBANKA_1138400
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PB001043.00.0, PB105593.00.0, PBANKA_113840
Orthologs PCHAS_1137900 , PF3D7_1362400 , PKNH_1108900 , PVP01_1109600 , PVX_115075 , PY17X_1139800
Google Scholar Search for all mentions of this gene