Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
PlasmoGEM (Barseq) Imported from PlasmoGEM
P. falciparum 3D7
Refractory
28784333
SLI
Theo Sanderson, Wellcome Trust Sanger Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) Imported from PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Live cell microscopy images of the endogenously GFP-tagged candidate PF3D7_0629200 (PFF1415c DnaJ protein) show PV location. The top microscopy panel row shows a ring and a trophozoite stage, the middle row shows a schizont and the bottom row shows a late schizont. The protein is not expressed in ring stages but in trophozoites and showed a signal in the parasite periphery consistent with a PV location. In addition pronounced foci in close proximity of the nucleus weredetected, suggesting some protein remaining in the secretory pathway. It is therefore likely a protein found within the parasite and peripherally attached at the outer face of the PPM or the inner face of the PVM of trophozoitesKhosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T. Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID. Int J Med Microbiol. 2017 Jul 27. [Epub ahead of print]

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Properties of proteins conserved , DnaJ and 11. (A) Saponin lysis during microscopy of a cell expressing PF11_0246. The identical infected RBC is shown before (top row) and after (bottom row) lysis with saponin. (B) Released merozoites of parasites expressing PF11_0246 show peripheral fluorescence (arrowhead) in addition to a prominent focus within the parasite. Saponin treatment did not release the PV fluorescence (A). In some merozoites a peripheral staining was detected after their release from the host cell, indicating that this protein may be integral to the PPM (B).(C,D) Saponin lysis during microscopy of a cell expressing DnaJ (C) or candidate 11; Saponin lysis of living parasites showed that the staining around the parasite was unaffected by lysis of the PVM. (D). The identical infected RBC is shown before (top rows) and after (bottom rows) lysis with saponin. Fluorescence around the parasite vanished after permeabilisation of the PVM. DIC, differential interference contrast; size bars: 5 mm.Khosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T. Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID. Int J Med Microbiol. 2017 Jul 27. [Epub ahead of print] PMID: 28784333

See original on MMP

More information

PlasmoDB PBANKA_1127800
GeneDB PBANKA_1127800
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PB001516.02.0, PB300280.00.0, PBANKA_112780
Orthologs PCHAS_1127300 , PF3D7_0629200 , PKNH_1120300 , PVP01_1120300 , PVX_114560 , PY17X_1129300
Google Scholar Search for all mentions of this gene