Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-1105 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Difference from wild-type
RMgm-1105
No detailed analyses of the growth(cycle) of mutant parasites. Infected Balb/c mice had lower parasitemias and prolonged survival time.
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1105
Reduced (2x) oocyst production
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Samples from a synchronized P. falciparum culture were collected at different time points during the asexual stages of parasite development, and used for determining expression of plasmoDJ1 by IFA using anti-PfDJ1 antibodies. The images of indicated stages show the presence of plasmoDJ1-specific signal in parasites (DJ1), nucleic acid staining (DAPI), brightfield showing parasite and the infected erythrocyte boundaries (Bright) and the merged of all three images (Merged). The signal appears to be diffusely distributed throughout the parasite in all stages, indicating expression of plasmoDJ1. Singhal N, Atul, Mastan BS, Kumar KA, Sijwali PS. Genetic ablation of plasmoDJ1, a multi-activity enzyme, attenuates parasite virulence and reduces oocyst production. Biochem J. 2014 461(2):189-203.

See original on MMP

PlasmoDJ1 is expressed throughout the asexual erythrocytic stages. Samples from a synchronized P. falciparum culture were collected at different time points during the asexual stages of parasite development, and used for determining expression of plasmoDJ1 by IFA using anti-PfDJ1 antibodies. The images of indicated stages show the presence of plasmoDJ1-specific signal in parasites (DJ1), nucleic acid staining (DAPI), bright field showing parasite and the infected erythrocyte boundaries (Bright) and the merged of all three images (Merged). The signal appears to be diffusely distributed throughout the parasite in all stages, indicating expression of plasmoDJ1.Singhal N, Atul, Mastan BS, Kumar KA, Sijwali PS. Genetic ablation of plasmoDJ1, a multi-activity enzyme, attenuates parasite virulence and reduces oocyst production. Biochem J. 2014 461(2):189-203.

See original on MMP

More information

PlasmoDB PBANKA_1126200
GeneDB PBANKA_1126200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PB001025.01.0, PBANKA_112620
Orthologs PCHAS_1125700 , PF3D7_0627500 , PKNH_1122300 , PVP01_1122000 , PVX_114480 , PY17X_1127700
Google Scholar Search for all mentions of this gene