Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-134 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-134 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-134 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-134
Mutants showed normal exflagellation numbers and developed into mature ookinetes in vitro and in the midgut of A. stephensi mosquitoes. Ookinetes did not show any morphological differences from wild-type parasites. Mutant ookinetes cannot invade midgut epithelial cells as was shown by light microscopic analysis. Ookinetes cannot rupture the cell membrane of the midgut epithelial cell as was shown by electron microscopic analysis. Analysis of ookinete invasion/traversal showed that wild type ookinetes had invaded the midgut epithelium, and over half of them were attached to the basal lamina. The epithelial cells initially invaded by ookinetes were heavily damaged. They were characterized by high electron density staining, protrusion from the epithelium, absent or scant microvilli, and degeneration of cytoplasmic organelles such as mitochondria. In contrast, mutant ookinetes were found neither in the cytoplasm of the midgut epithelial cell nor beneath the basal lamina. All of the mutant ookinetes were located outside the epithelium, and over the half of them adhered to the membrane of the epithelial cell, attaching their apical tip to epithelial cell surface. The epithelial cell surface where the ookinete attached was invaginated toward the cytoplasm, but no apparent breakage was observed in the extended cell membrane. The attached epithelial cells were kept intact, as shown by staining of normal electron density, dense microvilli, retention of the complex structure of the basal membrane labyrinth, and intact cytoplasmic organelles.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-134
No oocysts are formed. Mutant ookinetes cannot invade and traverse midgut epithelial cells (see phenotype 'Fertilization and ookinete').
Imported from RMgmDB

More information

PlasmoDB PBANKA_0824200
GeneDB PBANKA_0824200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAOP, PB000936.01.0, PB301406.00.0, PBANKA_082420
Orthologs PCHAS_0824500 , PF3D7_0923300 , PKNH_0721300 , PVP01_0721800 , PVX_099575 , PY17X_0827500
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