Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-350 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-350 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-350
Normal numbers of male and female gametocytes are produced. Male and female gamete formation is normal (escape from host red blood cell, formation of 8 motile male gametes). Male gametes are strongly affected in their fertility, resulting in nearly complete inhibition of ookinete formation in vitro (>0.99%). Motile males fail to attach to and penetrate female gametes.Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-350
Male gametes are strongly affected in their fertility, resulting in nearly complete inhibition of ookinete formation in vitro (>0.99%); Motile males fail to attach to and penetrate female gametes.Mutant female gametes are fertile as shown by cross-fertilization with wild type male gametes.Mutant parasites produce low numbers of ookinetes in vivo (Anopheles stephensi). See also 'Additional remarks phenotype'.
Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-350 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-350 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-350 Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Location of PPLP2 in P. falciparum gametocytes during activation. A. Cultures of gametocytes before activation, and at 7 and 15 min post-activation, were labelled with antiserum B directed against PPLP2 (green) and counterstained with (A) anti-Pfs230 antisera (red) or (B) anti-Pfs25. The corresponding differential interference contrast (DIC) images are shown. Note that mature P. falciparum gametocytes have a banana-like shape but round up during activation. Bar, 5 μm (A) and 2 μm (B). Deligianni E, Morgan RN, Bertuccini L, Wirth CC, Silmon de Monerri NC, Spanos L, Blackman MJ, Louis C, Pradel G, Siden-Kiamos I. A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes. Cell Microbiol. 2013 15(8):1438-55 PMID:

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Immunofluorescence assays of P. falciparum gametocytes at 15 min post-activation showed PPLP2 labelling (green) in association with shed membranes (indicated by arrows). The exflagellating microgametocyte is highlighted by labelling of Pfs25 (red). Arrowheads indicate microgametes. The corresponding differential interference contrast (DIC) images are shown. Bar, 5 μm.Deligianni E, Morgan RN, Bertuccini L, Wirth CC, Silmon de Monerri NC, Spanos L, Blackman MJ, Louis C, Pradel G, Siden-Kiamos I. A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes. Cell Microbiol. 2013 15(8):1438-55

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Immunofluorescence microscopy of P. falciparum merozoites and sexual stages. Upper panel A: fresh merozoite preparations were treated with anti-PfP0N (a, N-terminal), anti-PfP0C (b, C-terminal) and anti-MSP1 antibodies, each at a dilution of 1:50. Bound antibodies were visualized with anti-rabbit IgG-FITC secondary antibodies. The left hand panels show the bright field, and the right hand panels show the immunofluorescence. Bar corresponds to 1 mm. Lower Panel B: co-staining immunofluorescence analysis of macro gametes and red cell-free gametocytes. Panel a (bright field); b and c (corresponding immunofluorescent images) of a clump of two gametes and one gametocyte. Panel d (bright field), e and f (corresponding immunofluorescent images) of a single gametocyte. Panel g and i are the bright fields showing a gametocyte, while h andj are the corresponding immunofluorescent images, respectively.Panels a–f show the parasites treated with rabbit anti-PfP0N and Mab anti-Pfs230 [21]; g and h are gametocytes treated with rabbit pre-immune sera, and i and j are gametocytes treated with Mab anti-Pfg27, an internal marker of sexual stages . Anti-PfP0N was visualized with anti- rabbit IgG rhodamine secondary antibodies (b, e), while the monoclonal antibodies were visualized with anti-mouse IgG-FITC secondary antibodies (c, f and j). Rabbit pre-immune serum (panel h) was visualized with anti-rabbit IgG-FITC. All antibodies were used at 1:100 dilution. Bar corresponds to 5 mm.Chatterjee S, Singh S, Sohoni R, Kattige V, Deshpande C, Chiplunkar S, Kumar N, Sharma S. Characterization of domains of the phosphoriboprotein P0 of Plasmodium falciparum. Mol Biochem Parasitol. 2000 107(2):143-54.

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Protein expression profile of selected P. falciparum proteases. IFAs using polyclonal mouse antisera directed against 12 of the proteases was used to demonstrate protein expression in the asexual blood stages (R, ring; T, trophozoite; iS, immature schizont; mS, mature schizont) and gametocytes (GC stages III, IV and V) as well as in macrogametes (MaG) at 20 min post-activation (in green). The asexual and the sexual blood sstages were visualized via immunolabelling with polyclonal rabbit antisera against PfMSP1 and Pfs230, respectively (in red). Hoechst 33342 staining was used to highlight the parasite nuclei (in blue). Bar, 5 μm. Results are representative of three to four independent experiments.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]

See original on MMP

Protein expression profile of selected P. falciparum proteases. IFAs using polyclonal mouse antisera directed against 12 of the proteases was used to demonstrate protein expression in the asexual blood stages (R, ring; T, trophozoite; iS, immature schizont; mS, mature schizont) and gametocytes (GC stages III, IV and V) as well as in macrogametes (MaG) at 20 min post-activation (in green). The asexual and the sexual blood sstages were visualized via immunolabelling with polyclonal rabbit antisera against PfMSP1 and Pfs230, respectively (in red). Hoechst 33342 staining was used to highlight the parasite nuclei (in blue). Bar, 5 μm. Results are representative of three to four independent experiments.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]

See original on MMP

Protein expression profile of selected P. falciparum proteases. IFAs using polyclonal mouse antisera directed against 12 of the proteases was used to demonstrate protein expression in the asexual blood stages (R, ring; T, trophozoite; iS, immature schizont; mS, mature schizont) and gametocytes (GC stages III, IV and V) as well as in macrogametes (MaG) at 20 min post-activation (in green). The asexual and the sexual blood sstages were visualized via immunolabelling with polyclonal rabbit antisera against PfMSP1 and Pfs230, respectively (in red). Hoechst 33342 staining was used to highlight the parasite nuclei (in blue). Bar, 5 μm. Results are representative of three to four independent experiments.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]

See original on MMP

Protein expression profile of selected P. falciparum proteases. IFAs using polyclonal mouse antisera directed against 12 of the proteases was used to demonstrate protein expression in the asexual blood stages (R, ring; T, trophozoite; iS, immature schizont; mS, mature schizont) and gametocytes (GC stages III, IV and V) as well as in macrogametes (MaG) at 20 min post-activation (in green). The asexual and the sexual blood sstages were visualized via immunolabelling with polyclonal rabbit antisera against PfMSP1 and Pfs230, respectively (in red). Hoechst 33342 staining was used to highlight the parasite nuclei (in blue). Bar, 5 μm. Results are representative of three to four independent experiments.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]

See original on MMP

Protein expression profile of selected P. falciparum proteases. IFAs using polyclonal mouse antisera directed against 12 of the proteases was used to demonstrate protein expression in the asexual blood stages (R, ring; T, trophozoite; iS, immature schizont; mS, mature schizont) and gametocytes (GC stages III, IV and V) as well as in macrogametes (MaG) at 20 min post-activation (in green). The asexual and the sexual blood sstages were visualized via immunolabelling with polyclonal rabbit antisera against PfMSP1 and Pfs230, respectively (in red). Hoechst 33342 staining was used to highlight the parasite nuclei (in blue). Bar, 5 μm. Results are representative of three to four independent experiments.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]

See original on MMP

Protein expression profile of selected P. falciparum proteases. IFAs using polyclonal mouse antisera directed against 12 of the proteases was used to demonstrate protein expression in the asexual blood stages (R, ring; T, trophozoite; iS, immature schizont; mS, mature schizont) and gametocytes (GC stages III, IV and V) as well as in macrogametes (MaG) at 20 min post-activation (in green). The asexual and the sexual blood sstages were visualized via immunolabelling with polyclonal rabbit antisera against PfMSP1 and Pfs230, respectively (in red). Hoechst 33342 staining was used to highlight the parasite nuclei (in blue). Bar, 5 μm. Results are representative of three to four independent experiments.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]

See original on MMP

Immunolabeling control of blood stage parasite samples. Non-immunized mouse sera (NMS) were used to immunolabel fixed samples of trophozoites, schizonts and mature gametocytes (GC) as well as of activated gametocytes (aGC) at 30 min post-activation (green). Schizonts were visualized by labelling with rabbit anti-MSP1 antisera and gametocytes were visualized by rabbit anti-Pfs230 antisera (red); nuclei were highlighted by Hoechst stain (blue). Bar, 5 µm. Results shown are representative for three independent experiments.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]

See original on MMP

More information

PlasmoDB PBANKA_0306100
GeneDB PBANKA_0306100
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PB000290.01.0, PB000403.00.0, PB000537.00.0, PB301126.00.0, PB301326.00.0, PB301329.00.0, PB404640.00.0, PBANKA_030610
Orthologs PCHAS_0308300 , PF3D7_0209000 , PKNH_0412100 , PVP01_0415800 , PVX_003905 , PY17X_0306700
Google Scholar Search for all mentions of this gene